WSSP-04 Lecture notes (includes text and figures in .pdf format)

This web page provides links to materials for the Waksman Student Scholars Summer Program - 2004. This information is intended for the students and faculty participating in the program during the 2004 summer and through the 2004-2005 academic year. This material is not intended for general public use and access is password protected. The material on these pages is provided for educational purposes only. Unauthorized use of this material is prohibited. If you wish to download the files or have comments or questions about the material please contact Sue Coletta, Waksman Institute, Rutgers University

The Directors of the WSSP-04 thank instructors of the Rutgers University Introduction to Research in Molecular Biology and Biochemistry course (694:315 and 447:315) for allowing us to use some of the figures and notes in the WSSP-04 lecture notes.

Chapter 1 (400k) - The Basics: Replication, Transcription, and Translation

Chapter 2 (500k) - Vectors: Plasmids, Phage, and Plasmid DNA preps

Chapter 3 (400k) - Restriction enzymes and PCR

Chapter 4 (800k) - Mapping: Determining the size of a plasmid insert

Chapter 5 (768k) - DNA Sequencing

Chapter 6 (550k) - The WSSP-04 Project: Genomic analysis of C. remanei

Chapter 7a (1.1MB) - Introduction to Bioinformatics and Step 1: Analyze the DNA sequence waveform

Chapter 7b (970k) - Bioinformatics Steps 2-3: Identify the ORF and perform a BLASTN

Chapter 7c (930k) - Bioinformatics Steps 4-6: Analysis of the BLASTN and identification of the match

Chapter 7d (3.9MB) - Bioinformatics Steps 7-8: WormBase, WormPD and Protein Structure

Chapter 7e - Bioinformatics Step 9: Analyzing the cDNA sequence for submission to the database

Chapter 8 (4.6MB) - RNAi