WSSP-06 Lecture and Lab Files
This web page provides links to materials for the Waksman Student Scholars Program-2006. This information is intended for the students and faculty participating in the program during the 2006 summer and through the 2006-2007 academic year. This material is not intended for general public use and access is password protected. The material on these pages is provided for educational purposes only. Unauthorized use of this material is prohibited. If you wish to download the files or have comments or questions about the material please contact Sue Coletta, Waksman Institute, Rutgers University.
WSSP-06 Lecture notes and figures
Ch. |
Topic | Notes (.doc) |
Figures (.ppt) |
| Introduction and overview of project | Ch.0.ppt (5M) | ||
1 |
Vectors: Plasmids, Phage, and Plasmid DNA preps | Ch. 1 272K | Ch1.ppt (284K) |
2 |
Libraries | Ch. 2 428K | Ch2.ppt (800K) |
3 |
Restriction enzymes and PCR | Ch. 3 348K | Ch3.ppt (760K) |
4 |
Mapping: Determining the size of a plasmid insert | Ch. 4 632K | Ch4.ppt (XM) |
5 |
DNA Sequencing, Logging onto DSAP | Ch. 5 1.9M | Ch5.ppt (XM) |
6 |
Sequence quality cropping | Ch. 6 2.4M | Ch6.ppt (XM) |
7 |
Editing the waveform | Ch. 7 1.1M | Ch7.ppt (XM) |
8 |
BLASTN (DNA vs DNA search) | Ch. 8 1.3M | Ch8.ppt (XM) |
9 |
BLASTX (Translated DNA vs Protein search) | Ch. 9 4.8M | Ch9.ppt (XM) |
10 |
Determining ORFs, BLASTP (Protein vs Protein search) | Ch. 10 604K | Ch10.ppt (XM) |
11 |
Literature Search | Ch. 11 1.1M | Ch11.ppt (XM) |
12 |
Analysis of XP sequences: determine overlap, construct contigs | Ch. 12 1.9M | Ch12.ppt (XM) |
13 |
Spanning the gap between SP and XP sequences | Ch. 13 XXK | Ch13.ppt (XM) |
14 |
Multiple Sequence alignment and structure analysis | Ch. 14 2.3M | Ch14.ppt (XM) |
WSSP-06 Lab protocols and notes
Lab |
Topic | .doc File (Size) |
1 |
Introduction to the WSSP laboratory | Lab 1 (120K) |
2 |
Pipeting and weighing | Lab 2 (360K) |
3 |
Sterile technique and setting up ON cultures | Lab 3 (92K) |
4 |
Plasmid DNA minipreps (Detailed protocol) | Lab 4 (168K) |
5 |
Plasmid DNA minipreps (Short protocol) | Lab 5 (88K) |
6 |
Restriction enzymes and PCR (Detailed protocol) | Lab 6 (92K) |
7 |
Restriction enzymes and PCR (Short protocol) | Lab 7 (92K) |
8 |
Preparing an agarose gel (Detailed protocol) | Lab 8 (40K) |
9 |
Running an agarose gel | Lab 9 (180K) |
10 |
DNA Sequencing | Lab 10 (40K) |
| Recipe for 1X TBE + EtBr | (.doc) | |
| Fall 2006 Supply list and instructions for experiments at the schools | (.doc) |